Thursday, April 23, 2015

GATTACA Reflection Questions

Questions about GATTACA!

these questions are for the movie Gattaca to see what we thought about it.



Describe the relationship between Vincent and Anton:
The relationship between Vincent an Anton is very problematic. Even when they were little kids Vincent would try to show Anton that he could do what he could. They would see who could swim out farther than the other and Anton would win all the time. so this would really annoy Vincent. so this made it a very problematic relationship.

Choose your favorite character from the film. Explain why you choose that person. Would you want to be that person? Why? Why not?:
The person I choose is Vincent. I choose Vincent because he showed people that he could do something that people like him could not do even tho he had to cheat to get to where he was. I think I would not what to be Vincent. The reson is that he has a hart problem and i don't think that i could have a hart problem because of the life style i live.

Picture yourself as either Vincent, Jerome, or Anton. Would you have acted the same or done things differently if you were in the same world as them?:
if i was jerome i would have not put my self throw all of that bull trying to help another guy trying to be me i would have said screw this and left.








Wednesday, April 8, 2015

From DNA to Proteins: Central Dogma Vocabulary

From DNA to Proteins: Key Terms



Read about and define the terms below. Develop and publish a concept map, flashcard set, or other creative visual aid to help yourself and others learn the connections between these ideas.


Anticodon:A sequence of three adjacent nucleotides located on one end of transfer RNA. It bounds to the complementary coding triplet of nucleotides in messenger RNA during translation phase of proton synthsis.


Codon:A set of three adjacent nucleotides, also called triplet, in nRNA that base-pair with the corresponding anticodn of tRNA molecule that carries a particular amino acid, hence, specifying the type and sequence of amino acids for protein synthesis.



Exon:1. The protein-coding region in the DNA.
2. The nucleic acid sequence in the DNA, or RNA transcript following genetic splicing.



Genetic Code:(Sciencemolecular biology) relationship between the sequence of bases in nucleic acid and the order of amino acids in the polypeptide synthesised from it. A sequenceof three nucleic acid bases (a triplet) acts as a codeword (codon) for one amino acid.


Intron:(molecular biology) A noncoding, intervening sequence of DNA within a gene that is transcribed into mRNA but is removed from the primary gene transcript and rapidly degraded during maturation of the RNA product


mRNA (messenger RNA):A type of RNA that carries the code or chemical blueprint for a specific protein. In the early stages of protein synthesis, the mRNA is synthesized from a DNA templateduring transcription.


Promoter:(genetics) A site in a DNA molecule at which RNA polymerase and transcription factors bind to initiate transcription of mRNA.



Protein-coding Gene:The boundaries of a protein-encoding geneare defined as the points at which transcription begins and ends. The core of thegene is the coding region, which contains the nucleotide sequence that is eventually translated into the sequence of amino acids in the protein.


Ribonucleic Acid (RNA):nucleic acid that is generally single stranded (double stranded in some viruses and siRNA), composed of repeating nucleotide units of ribose sugarphosphate group, and nitrogenous base.


RNA polymerase:Rna polymerase (RNAP or RNApol) is an enzyme that is responsible for making rna from a dna template. In all cells RNAP is needed for constructing rna chains from adna template, a process termed transcription. In scientific terms, RNAP is a nucleotidyl transferase that polymerizes ribonucleotides at the 3' end of an rna transcript. Rna polymerase enzymes are essential and are found in all organismscells, and many viruses.


Transcription:Transcription is the first step of gene expression, in which a particular segment of DNA is copied into RNA by the enzyme RNA polymerase. Both RNA and DNA are nucleic acids, which use base pairs of nucleotides as a complementary language that can be converted back and forth from DNA to RNA by the action of the correct enzymes. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, antiparallel RNA strand called a primary transcript. As opposed to DNA replication, transcription results in an RNA complement that includes the nucleotide uracil (U) in all instances where thymine (T) would have occurred in a DNA complement. Also unlike DNA replication where DNA is synthesized, transcription does not involve an RNA primer to initiate RNA synthesis.Although Transcription is nice.


Translation:A step in protein biosynthesis wherein the genetic code carried by mRNA is decoded to produce the specific sequence of amino acids in a polypeptide chain. The process follows transcription in which the DNA sequence is copied (or transcribed) into an mRNA.


tRNA (transfer RNA):RNA involved in protein synthesis, i.e. transporting specific amino acid to the ribosome to be added onto the growing polypeptide chain

Monday, April 6, 2015

DNA EXTRACTION FROM WHEAT GERM




DNA Extraction of Wheat Germ
Instructions:
  1. place 1 gram of raw wheat germ in a 50 ml test tube
  2. add 20 ml of hot tap water and mix constantly for 3 mins
  3. add 1 ml or a scant 1/4 teaspoon of detergent and mix gently every minute for 5 minutes. try not to creat foam
  4. use an eyedropper, pipette, of a pice of paper towel to remove any foam from the top of the solution
  5. tilt the test tube at an angel. SLOWLY pour 14 ml of alcohol down the side so that it forns a layer on to top of the water/wheat germ/detergent soultion. do not mix the two layers together. DNA precipitates at the water-alcohol interface ( the  boundary between the water and the alcholo). therefore, it is crucial to pour the alcohol very slowly so that it forms a layer on top of the water soultion. if the alcohol mixes with thw water, it will become too dilute and the DNA will not precipitate.
  6. let the test tube sit for a few minutes. white, stringy, filmy DNA will begin to appear where the water and alcohol meet. you will usuly see DNA precipitating fron the solution at the water-alcohol interface as soon as you pour in the alcohol. if yu let the preparation sit for 15 minutes or so the DNA will float to the top of the alcohol. you cam usually get more DNA to prectpaite from the solutio by using one of the DNA-collection tools ( such as a glass or paper clip hook) to gently lift the water solution up in the alcohol. this allows more DNA to come in contact with the alcohol and precipitate. you may find it helpful to pour the water/detergent soultion into a clean test tube, leaving behind the wheat germ, before adding the alcohol.
  7. finally use a glass or paper clip hook or a wooden stick to collect the DNA.
observations:

  1. what dose the wheat germ soup look like: it looks like italen dressing
  2.  how dose its appearance change as you add the detergent and swirl it in?: the mixter went from brow to yellow
  3. what do you think is happening at this step: the detrgent is destroying the membrane to get rid of the oils
  4. describe the appearance of the mixture just after you add the alcohol: the wheat is at the botton, yeelow in the middle and alcohol at the top
  5. what do you think is happening at this step: both types of DNA is mixing to make one type
  6. what do you obseruve at the water-alcohol interface: white goo stuff staterd to flot to the top of the test tube
  7. what doce DNA look like? it looks like a white clear goo.